Mass spectrometric investigation of cardiolipins and their. This exactive exacase is a part expanding storage and filing solution for all your needs. For information about the operating procedures for the exactive plus mass spectrometer, we recommend that you read the exactive plus. Genecraft labs trusted partner for laboratory solution. High resolution lcms for screening and quantitative analysis. The q exactive hf, a benchtop mass spectrometer with a. The exactive plus mass spectrometer is the ultimate solution for screening. The msdata was analyzed with maxquant software version 1.
View brochure download pdf file lr, 4mb download pdf file hr, 29mb office. Calibrating thermo exactive with the direct analysis in real. The q exactive a benchtop orbitrap mass spectrometer. Selecting the best q exactive orbitrap mass spectrometer scan. Thermo scientific q exactive plus orbitrap lcmsms system. If you change the file location later, the instrument may not recognize your file. Q exactive classic plus instruments were not tested and are not officially supported. Characterization of stress degradation products of fosamprenavir calcium using q exactive plus orbitrap and ltq xl mass spectrometers, and prediction of their toxicity using admet predictor.
Characterization of a novel orthomyxolike virus causing mass. Driver controls of the ma3100 are embedded within ms software package exactive plus, exactive plus emr, q exactive, and q exactive plus. Monoclonal antibody characterization on q exactive and oribtrap elite yi zhang, ph. Avmbiomed also provides solutions for questions that require targeted approaches to the proteome e. Who uses this guide this exactive plus software manual is intended for all personnel that. Intact monoclonal antibody characterization using a bench. Printed manuals exactive plus operating manual ion max and ion maxs api source hardware manual in addition to these printed manuals, other documents for the exactive plus mass spectrometer are. Now i have to generate the same report, but in pdf format. The orbitrap voltage is 5 kv as it is on the exactive and therefore higher than the 3. Dataindependent acquisition for the orbitrap q exactive hf. Fundamentals and advances of orbitrap mass spectrometry. Ms performance max creating solutions ms standards. Fundamentals and advances of orbitrap mass spectrometry hecht. Preferably, id like to use the excel file itself, because the datasets that i use to feed the excel files, are incomplete.
Enhanced analysis of native proteins and protein complexes with optional intact protein mode. Tune plus driver controls of the ma3100 are embedded within ms software package exactive plus, exactive plus emr, q exactive, and q exactive plus excellims visiontm control and visiontm. Download pdf file lr, 4mb download pdf file hr, 29mb office. To record the nmr spectrums for the degradation products, bruker 400 mhz. Ability to quantitate and confirm samples in the same analytical run for antibiotics in water samples. Selecting the best q exactive orbitrap mass spectrometer.
Shop online our large selection of bioinfomatics analysis and data analysis software. The transient length for the q exactive plus at 70k mass resolution mz 400 is 256 ms and increases linearly with mass resolution e. About this guide this exactive operating manual describes the modes of operation and principle hardware components of your exactive instrument. Exactive exacase multipart expanding file javascript seems to be disabled in your browser. Unpack the zip file in a new folder on the local hard drive of the. No further optimization of the lcms system was needed. Performance characteristics of an ap maldi ion source applied. Calibrating thermo exactive with the direct analysis in.
The ms analysis was performed on a thermo scientific q exactive plus benchtop high resolution mass spectrometer using electrospray ionization esi. Mass spectrometrybased proteomics using q exactive, a high. Tune plus driver controls of the ma3100 are embedded within ms software package exactive plus, exactive plus emr, q exactive, and q exactive plus excellims visiontm control and visiontm analysis ma3100 control and data acquisition software for compatible mass spectrometers outside of the exactive product line excellims corporation. This benchtop lcms system delivers highresolution, accuratemass hram data and fast fullscan capabilities to increase your sample through. Characterization of a novel orthomyxolike virus causing. Pdf introduction 120 words thermo scientific has recently introduced two new mass spectrometers ms. The q exactive plus hybrid quadrupoleorbitrap mass spectrometer takes quanfirmation to the next level with increased performance and new options that enhance applications from confident dmpk.
Individual retention times measured for representative compounds are within 0. The q exactive series mass spectrometer however provides a significantly greater mass resolving power up to 280,000 fwhm at mz 200 on the q exactive plus mass spectrometer. Exactive operating manual thermo fisher scientific. Identification, isolation and structureconfirmation of. Exactive exactive plus q exactive q exactive plus ltq ft ultra tsq access max tsq quantum discovery tsq quantum ultra tsq vantage tsq endura tsq quantiva creating solutions ltq xl hybrid calmix normal mass range, positive tfs200 creating solutions ltq velos hybrid calmix normal mass range, positive tfs210 creating solutions ltq hybrid calmix. Thermo fisher exactive plus emr orbitrop was used for hrms analysis of degradation products with x caliber software. Reference information provides information about various file types and other supplemental information.
The q exactive series mass spectrometer however provides a significantly greater mass resolving power up to 280,000 fwhm at mz 200 on the q exactive plus mass spectrometer and does not require spectral averaging for highconfidence data generation. Calibrating thermo exactive with the direct analysis in real time dart. Oct 14, 2012 modifications to an orbitrapbased mass spectrometer enable analysis of large protein complexes in nativelike states by mass spectrometry with very high sensitivity and mass resolution. Please note that this guide does not contain any safe ty notices and precautionary statements. Thermo scientific q exactive plus orbitrap lcmsms system characterize, quantify and confirm with unmatched confidence the thermo scientific q exactive plus benchtop lcmsms combines highperformance quadrupole precursor selection with highresolution, accuratemass hram orbitrap detection to deliver. Coupling irmaldesi with drift tube ion mobility, sitora. Ma3100 control and data acquisition software for compatible mass. We provide a wide range of solutions for products and services for research, education, quality control and testing field. Jun 03, 2011 the orbitrap voltage is 5 kv as it is on the exactive and therefore higher than the 3.
The q exactive also employs a 90 bent ion path from the source toward the mass analyzer in common with the exactive and in contrast to the ltq orbitrap instruments. The ion source is coupled in front of the heated capillary which is integrated in a stacked ring ion guide srig, also known as slens. The thermo scientific q exactive hybrid quadrupoleorbitrap mass spectrometer introduced the revolutionary concept of quanfirmation. I understand that i may be liable for a field service representatives travel or lodging expenses if they are una ble to carry out the installation on the pr escheduled date due to insufficient lab p reparation. Peptides were separated on a c18reversed phase column 15 cm long, 75. A variable temperature magnetic susceptibility measurement of a crystalline sample of 3 was done on a mpms 3 quantum design squid magnetometer.
Optimized instrument control electronics in conjunction with patented linac collision cell technology delivers ultrafast acquisition rates of up to 100 msms per cycle for ida. Diclofenac is a monocarboxylic acid consisting of phenylacetic acid having a 2,6dichlorophenylamino group at the 2position. Captopril is an angiotensinconverting enzyme ace inhibitor used in the therapy of hypertension and heart failure. Instrument setup window, and the exactive plus tune window. Screen, identify and quantify compounds in complex samples rapidly and with confidence using the thermo scientific exactive plus orbitrap mass spectrometer. Red line creating solutions specifically designed for thermo mass. Intact monoclonal antibody characterization using a benchtop orbitrap mass spectrometer zhiqi hao thermo fisher scientific. In addition to these printed manuals, other documents for the. The q exactive hf, a benchtop mass spectrometer with a prefilter. Exactive plus inclusion mass list file used by the exactive plus method for the ddaif scan triggering as described previously. Calibration solutions for thermo mass spectrometers red line creating solutions. Focus 200 plus alarm system manual tyco integrated security k36931v4 811 rev c.
For information about performing these tasks, refer to the exactive plus operating manual, the exactive plus software manual or the exactive plus tune help, and the ion max and ion maxs api source hardware manual. Captopril is associated with a low rate of transient serum aminotransferase elevations. Essential tool for molecular weight identification for small molecules, peptides, proteins, ect. Then click on fileexport and give it a path and file name, this will save the template as a csv file. It has a role as a nonnarcotic analgesic, an antipyretic, an ec 1.
Preferably, id like to use the excel file itself, because the datasets that i use to feed the excel files, are. Doclive free unlimited document files search and download. Thermo scientific q exactive plus orbitrap lcmsms system characterize, quantify and confirm with unmatched confidence the thermo scientific q exactive plus benchtop lcmsms combines high. The cellular response to lanthanum is substrate specific. Amino acids are key substrates to escherichia coli bw251. With more ions produced, better ion focusing, improved ion reflection and maximized detection, you will experience the performance edge with the tripletof 6600 system. Printed manuals exactive plus operating manual ion max and ion maxs api source hardware manual in addition to these printed manuals, other documents for the exactive plus mass spectrometer are available as pdf files on the data system computer. Data file of a deep proteome analysis of the prefrontal. For information about performing these tasks, refer to the. For information about the operating procedures for the exactive plus mass spectrometer, we recommend that you read the exactive plus operating manual in its entirety. High resolution lcms for screening and quantitative. Then click on file export and give it a path and file name, this will save the template as a csv file. Monoclonal antibody characterization on q exactive and.
Reference information provides information about various file types and other. The quadrupole orbitrap mass spectrometer q exactive made a powerful proteomics instrument available in a benchtop format. The thermo scientific q exactive plus takes quanfirmation to the next level with increased performance and new. The q exactive hfx also enables rapid phosphoproteomics with routine analysis of more than five thousand phosphopeptides with short singleshot 15minute lcmsms measurements, or 16,700. Supporting information ultraviolet photodissociation of esi. Related documentation in addition to this guide, thermo fisher scientific provides the following documents for the q exactive plus mass. Orbitrap signal decay can be overcome by enhancing the amplitude of the signal. High resolution full scan ms and datadependent top 3 msms data were collected in a datadependent fashion at a resolving power of 70,000 and 17,500 fwhm mz 200 with polarity switching. Increasing speed of uhplcms analysis using singlestage. Ever since the discovery of the first rare earth element reedependent enzyme, the physiological role of lanthanides has become an emerging field of research due to the environmental implications and. Exactive is a member of the thermo scientific fa mily of advanced mass spectrometer ms detectors.
Dosing syringe extractables analysis using benchtop orbitrap. Exactive plus lcms produces fast, reproducible results without. Extracted chromatograms for a selection of compounds figure 9. Resulting peptides were separated with agilent 1200 nanoflow system agilent technologies and then injected to q exactive plus thermo fisher scientific mass spectrometer with nanoelectrospray ionization source proxeon. Top reasons to upgrade to a q exactive hfx hybrid quadrupole. The q exactive plus hybrid quadrupoleorbitrap mass spectrometer takes quanfirmation to the next level with increased performance and new options that enhance applications from confident dmpk qualquan screening studies to characterization of intact monoclonal antibodies. Apr 05, 2016 the samples were trypsinized and analyzed by liquid chromatographytandem mass spectrometry lcmsms on q exactive plus thermo scientific. Who uses this guide this exactive plus software manual is intended for all personnel that need to perform measurements with the exactive plus mass spectrometer, especially the key. Manuals for the delivered ion sources and other software. Q exactive plus hybrid quadrupoleorbitrap mass spectrometer. Exactive plus mass spectrometer are available as pdf files on the data system computer. The data system computer with the exactive plus tune software is switched on and the mass spectrometer is placed in standby mode.
Read this first about this guide ii q exactive plus software manual pn 44780, revision a thermo scientific chapter 6. The biomass mix was lysed and digested with trypsin. This guide is intended for all personnel who operate the q exactive mass spectrometer. Exactive plus orbitrap lcms system thermo fisher scientific. Thermo scientific exactive plus benchtop fullscan orbitrap mass. The quantitation of hram data using tracefinder software from the exactive plus orbitrap instrument. This q exactive quickstart guide gives an introduction on setting up and using the thermo scientific q exactive mass spectrometer. Lchrms for quantitative bioanalysis in the regulated. The samples were trypsinized and analyzed by liquid chromatographytandem mass spectrometry lcmsms on q exactive plus thermo scientific. Bottom lipid extract spectra obtained on the imqtof platform, with annotations as noted in figure 4 of the main text. Tripletof 6600 high resolution accurate mass system sciex. Heck at utrecht university, who used the exactive plus emr mass. Maldi mass spectra were obtained on a bruker impacttm ii maldi mass spectrometer.
The thermo scientific q exactive plus benchtop orbitrap mass spectrometer driver has. Pt genecraft labs, established in 2006, is a leading life science, analytical and laboratory instruments, reagents and consumables distributor in indonesia. A read is counted each time someone views a publication summary such as the title, abstract, and list of authors, clicks on a figure, or views or downloads the fulltext. Data file of a deep proteome analysis of the prefrontal cortex in aged mice with progranulin deficiency or neuronal overexpression of progranulin. Therefore the hplc system was hyphenated to a q exactive plus or exactive orbitrap mass spectrometer.
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